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4GGM

Structure of LpxI

Experimental procedure
Experimental methodMAD
Source typeSYNCHROTRON
Source detailsALS BEAMLINE 8.3.1
Synchrotron siteALS
Beamline8.3.1
Temperature [K]100
Detector technologyCCD
Collection date2009-10-16
DetectorADSC QUANTUM 315r
Wavelength(s)1.11
Spacegroup nameI 41 2 2
Unit cell lengths74.286, 74.286, 364.644
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution45.513 - 2.897
R-factor0.2186
Rwork0.216
R-free0.27160
Structure solution methodSAD
RMSD bond length0.008
RMSD bond angle1.312
Data reduction softwareMOSFLM
Data scaling softwareSCALA
Phasing softwarePHASER
Refinement softwarePHENIX (1.7.3_928)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]45.0003.189
High resolution limit [Å]2.9002.897
Number of reflections11932
Completeness [%]99.699.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.7293100 mM MES pH 5.7, 3-7% w/v PEG 6000, 2.5% v/v glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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