4GGM
Structure of LpxI
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.11 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 74.286, 74.286, 364.644 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.513 - 2.897 |
R-factor | 0.2186 |
Rwork | 0.216 |
R-free | 0.27160 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.312 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 3.189 |
High resolution limit [Å] | 2.900 | 2.897 |
Number of reflections | 11932 | |
Completeness [%] | 99.6 | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | 100 mM MES pH 5.7, 3-7% w/v PEG 6000, 2.5% v/v glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |