4GGG

Crystal structure of V66A/L68V CzrA in the Zn(II)bound state.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	113
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 2 21
Unit cell lengths	46.286, 50.198, 85.877
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.669 - 1.998
R-factor	0.1778
Rwork	0.173
R-free	0.22430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r1v
RMSD bond length	0.005
RMSD bond angle	0.803
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.669	2.030
High resolution limit [Å]	1.998	2.000
Number of reflections	13543
Completeness [%]	91.5	94.2
Redundancy		5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	293	PEG 8000, NaCl, Ches, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293 K, temperature 293.K