4GG8
Immune Receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.956591 |
| Spacegroup name | P 3 |
| Unit cell lengths | 124.562, 124.562, 61.098 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.990 - 3.200 |
| R-factor | 0.265 |
| Rwork | 0.261 |
| R-free | 0.30800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gg6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.049 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.072 | 0.389 |
| Number of reflections | 15462 | |
| <I/σ(I)> | 14.6 | 2.2 |
| Completeness [%] | 87.2 | 89.7 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 3.8M SODIUM FORMATE, 0.1M TRIS PH 7.5, 5% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
