4GEZ
Structure of a neuraminidase-like protein from A/bat/Guatemala/164/2009
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-25 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 113.325, 113.331, 114.305 |
| Unit cell angles | 81.47, 81.55, 67.72 |
Refinement procedure
| Resolution | 29.884 - 2.500 |
| R-factor | 0.2207 |
| Rwork | 0.218 |
| R-free | 0.26160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.171 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 174667 | |
| <I/σ(I)> | 8.2 | 2 |
| Completeness [%] | 97.7 | 92.2 |
| Redundancy | 2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 293 | 0.8M ammonium acetate, 20% PEG 4600, 0.1M MES, pH 6.5, microbatch, temperature 293K |
