4GEZ
Structure of a neuraminidase-like protein from A/bat/Guatemala/164/2009
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-25 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 113.325, 113.331, 114.305 |
Unit cell angles | 81.47, 81.55, 67.72 |
Refinement procedure
Resolution | 29.884 - 2.500 |
R-factor | 0.2207 |
Rwork | 0.218 |
R-free | 0.26160 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.171 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 174667 | |
<I/σ(I)> | 8.2 | 2 |
Completeness [%] | 97.7 | 92.2 |
Redundancy | 2 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.5 | 293 | 0.8M ammonium acetate, 20% PEG 4600, 0.1M MES, pH 6.5, microbatch, temperature 293K |