4GEI
N-terminal domain of VDUP-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.250, 76.280, 31.030 |
| Unit cell angles | 90.00, 92.65, 90.00 |
Refinement procedure
| Resolution | 30.130 - 1.500 |
| R-factor | 0.18198 |
| Rwork | 0.180 |
| R-free | 0.20328 |
| Structure solution method | SAD |
| Starting model (for MR) | A MODEL OBTAINED FROM EXPERIMENTAL PHASING BY SAD USING PT SOAKED CRYSTALS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.817 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.062 | 0.672 |
| Number of reflections | 36857 | |
| <I/σ(I)> | 16.7 | 2.6 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 7.2 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 7.15 | 277 | 25mM Hepes, 1 mM DTT, pH 7.15, batch, temperature 277K |
