4GE2
Crystal structure of human protein tyrosine phosphatase PTPN9 (MEG2) complex with compound 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210r |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.982, 57.747, 66.544 |
| Unit cell angles | 77.33, 78.28, 80.27 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| Rwork | 0.189 |
| R-free | 0.22470 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2pa5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.149 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.240 |
| Rmerge | 0.147 |
| Number of reflections | 105301 |
| Completeness [%] | 68.6 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 25% PEG3350, 0.2 M potassium thiocyanate, 10% ethylene glycol, 0.1 M Bis-Tris propane, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
