4GDO
Structure of a fragment of the rod domain of plectin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR-H |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-04-01 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.620, 35.870, 75.690 |
Unit cell angles | 90.00, 94.11, 90.00 |
Refinement procedure
Resolution | 45.194 - 1.700 |
R-factor | 0.2148 |
Rwork | 0.213 |
R-free | 0.24840 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.008 |
RMSD bond angle | 0.870 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | Arcimboldo |
Refinement software | PHENIX ((phenix.refine: dev_1117)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.000 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 23087 | |
<I/σ(I)> | 3.52 | |
Completeness [%] | 85.3 | 68.9 |
Redundancy | 3.1 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 0.1M sodium acetate, 2.2M sodium chloride, 0.2M lithium sulfate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |