4GDA
Circular Permuted Streptavidin A50/N49
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-06-15 |
Wavelength(s) | 0.78 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 46.809, 94.027, 104.279 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.450 - 1.000 |
R-factor | 0.1195 |
Rwork | 0.118 |
R-free | 0.14320 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.011 |
RMSD bond angle | 1.574 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 14.456 | 14.456 | 0.990 |
High resolution limit [Å] | 0.970 | 2.630 | 0.970 |
Rmerge | 0.076 | 0.048 | 0.997 |
Number of reflections | 135318 | ||
<I/σ(I)> | 20.671 | ||
Completeness [%] | 99.8 | 98.1 | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 25% saturated ammonium sulfate, 0.1 M Tris-chloride, 0.2 M lithium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |