4GD4
Crystal Structure of JMJD2A Complexed with Inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-07-11 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.91730 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 100.750, 150.030, 57.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 83.640 - 2.330 |
R-factor | 0.182 |
Rwork | 0.180 |
R-free | 0.21820 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.030 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.640 | 2.390 |
High resolution limit [Å] | 2.330 | 2.330 |
Rmerge | 0.080 | 0.628 |
Number of reflections | 37798 | |
<I/σ(I)> | 12.8 | 2.7 |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 5.4 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 277 | 0.2 M NaF, 20% w/v PEG 3350, 0.1 M Bis Tris Propane, pH 7.5, VAPOR DIFFUSION, temperature 277K |