4GD0
tRNA-guanine transglycosylase Y106F, C158V mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 113.15 |
Detector technology | CCD |
Collection date | 2010-10-21 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 91.428, 64.986, 70.203 |
Unit cell angles | 90.00, 96.10, 90.00 |
Refinement procedure
Resolution | 16.275 - 1.290 |
R-factor | 0.1651 |
Rwork | 0.163 |
R-free | 0.18550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3GEV |
RMSD bond length | 0.005 |
RMSD bond angle | 1.060 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.310 |
High resolution limit [Å] | 1.290 | 1.290 |
Number of reflections | 99752 | |
<I/σ(I)> | 22.1 | 3.02 |
Completeness [%] | 97.1 | 81.5 |
Redundancy | 2.7 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | 100 mM Tris, 1 mM DTT, 10 % DMSO, 12% PEG 8000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K |