4GCX
tRNA-guanine transglycosylase Y106F, C158V, V233G mutant in complex with preQ1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 113.15 |
Detector technology | CCD |
Collection date | 2010-12-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 91.189, 65.053, 70.616 |
Unit cell angles | 90.00, 96.14, 90.00 |
Refinement procedure
Resolution | 24.732 - 1.420 |
R-factor | 0.1342 |
Rwork | 0.131 |
R-free | 0.16110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3GEV |
RMSD bond length | 0.006 |
RMSD bond angle | 1.108 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.440 |
High resolution limit [Å] | 1.420 | 1.420 |
Number of reflections | 76733 | |
<I/σ(I)> | 16.5 | 2.2 |
Completeness [%] | 99.0 | 96.2 |
Redundancy | 3.1 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | 100 mM Tris, 1mM DTT, 10% DMSO, 12% PEG 8000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K |