4GCV
Structure of a Putative transcription factor (PA1374)from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Detector technology | CCD |
| Collection date | 2011-11-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97959 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.897, 74.448, 230.492 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 29.720 - 2.300 |
| R-factor | 0.21763 |
| Rwork | 0.214 |
| R-free | 0.28695 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.771 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.720 | |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 96489 | |
| Completeness [%] | 99.4 | 99.06 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 298 | 0.1M NaCl, 5.9% PEG3350, 0.1M Phosphate-citrate pH3.6 25% Glycerol, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
