4GCN

N-terminal domain of stress-induced protein-1 (STI-1) from C.elegans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	37.212, 64.199, 124.933
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.900 - 1.850
R-factor	0.1803
Rwork	0.178
R-free	0.23290
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.676
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.900	50.000	1.880
High resolution limit [Å]	1.850	5.020	1.850
Rmerge	0.079	0.056	0.585
Number of reflections	25569
<I/σ(I)>	9.2		2.37
Completeness [%]	97.0	99.4	74
Redundancy	7.6	7.5	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	0.2 M sodium chloride, 0.1 M Bis-Tris buffer, 25% PEG-3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K