4GC8

Crystal structure of FliM middle domain from H. pylori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 31
Unit cell lengths	91.267, 91.267, 57.086
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.846 - 2.200
R-factor	0.1787
Rwork	0.175
R-free	0.23730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.117
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.200
Number of reflections	26406

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	0.1M HEPES, 25% PEG2250, 20mM Sodium Bromide, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K