4GBV
Crystal Structure of AMP complexes of Porcine Liver Fructose-1,6-bisphosphatase Mutant A54L with 1,2-ethanediol as Cryo-protectant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | Cu FINE FOCUS |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 53.690, 82.506, 166.284 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.100 - 2.900 |
R-factor | 0.1803 |
Rwork | 0.177 |
R-free | 0.24270 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.545 |
Data reduction software | d*TREK (9.7L) |
Data scaling software | d*TREK (9.7L) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 32.100 | 32.100 | 3.000 |
High resolution limit [Å] | 2.900 | 6.230 | 2.900 |
Rmerge | 0.122 | 0.061 | 0.295 |
Total number of observations | 2471 | 2376 | |
Number of reflections | 8010 | ||
<I/σ(I)> | 6.6 | 13 | 3 |
Completeness [%] | 93.7 | 86.6 | 96.8 |
Redundancy | 2.96 | 3.05 | 2.89 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 7.5 | 298 | PEG 3350, t-butyl alcohol, 1,2-ethanediol, pH 7.5, hanging drop, temperature 298K |