4GBT
Structural characterization of H-1 Parvovirus: comparison of infectious virions to replication defective particles
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0895 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 255.200, 350.100, 272.100 |
| Unit cell angles | 90.00, 90.12, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.200 |
| Rwork | 0.256 |
| R-free | 0.25920 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.366 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 479478 | |
| <I/σ(I)> | 4.9 | 1.3 |
| Completeness [%] | 61.3 | 58.3 |
| Redundancy | 1.7 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 10 mM Tris-HCl, 150 mM NaCl, 8mM CaCl2.2H2O and 3% PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
