4GBF
Crystal structure of the C-terminal domain of gp131 from bacteriophage phiKZ
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-05-27 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.449, 86.353, 97.362 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.180 - 1.949 |
R-factor | 0.1625 |
Rwork | 0.161 |
R-free | 0.19860 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.887 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELX (C/D/E) |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.680 | 2.070 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 49414 | |
<I/σ(I)> | 15.37 | 4.1 |
Completeness [%] | 99.6 | 97.3 |
Redundancy | 9.2 | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 25% PEG3350, 0.25M magnesium chloride, 0.1M BisTris, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |