4GBF

Crystal structure of the C-terminal domain of gp131 from bacteriophage phiKZ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-05-27
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	79.449, 86.353, 97.362
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.180 - 1.949
R-factor	0.1625
Rwork	0.161
R-free	0.19860
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.887
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHELX (C/D/E)
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.680	2.070
High resolution limit [Å]	1.950	1.950
Number of reflections	49414
<I/σ(I)>	15.37	4.1
Completeness [%]	99.6	97.3
Redundancy	9.2	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	25% PEG3350, 0.25M magnesium chloride, 0.1M BisTris, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K