4GBA

DCNL complex with N-terminally acetylated NEDD8 E2 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	77
Detector technology	CCD
Collection date	2011-01-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97910
Spacegroup name	P 1 21 1
Unit cell lengths	83.465, 44.578, 101.216
Unit cell angles	90.00, 103.30, 90.00

Refinement procedure

Resolution	29.085 - 2.400
R-factor	0.1956
Rwork	0.194
R-free	0.22730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.032
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.100	30.000	2.490
High resolution limit [Å]	2.400	5.160	2.400
Number of reflections	28812
Completeness [%]	99.7	100	97.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	crystals in 2.28M sodium malonate, seeded into 2.05M sodium malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K