4GB5
Crystal structure of Kfla4162 protein from Kribbella flavida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-28 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 63 |
| Unit cell lengths | 57.948, 57.948, 81.605 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.974 - 1.550 |
| R-factor | 0.1458 |
| Rwork | 0.144 |
| R-free | 0.17980 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.391 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine: dev_1096)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.092 | 0.930 |
| Number of reflections | 22441 | |
| <I/σ(I)> | 24.3 | 2.1 |
| Completeness [%] | 99.3 | 98.6 |
| Redundancy | 7.5 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 289 | 0.2 M NaCl, 0.1 M NaH2PO4/K2HPO4, pH 6.2, 50% PEG200, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
