4GB3
Human coxsackievirus B3 strain RD coat protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-07 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 296.638, 296.638, 813.215 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.740 |
| R-factor | 0.33 |
| Rwork | 0.330 |
| R-free | 0.33900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cov |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.840 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.127 | 0.488 |
| Number of reflections | 343901 | |
| <I/σ(I)> | 8.75 | 2.97 |
| Completeness [%] | 96.9 | 98 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 298 | 2 M ammonium sulfate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
