4GA0
Structure of the N-terminal domain of Nup358
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-01-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 59.219, 82.768, 29.543 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.161 - 1.150 |
R-factor | 0.2157 |
Rwork | 0.214 |
R-free | 0.23930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ga2 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.123 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.200 | 1.190 |
High resolution limit [Å] | 1.150 | 1.150 |
Number of reflections | 50246 | |
<I/σ(I)> | 25.4 | 1.7 |
Completeness [%] | 96.7 | 76.6 |
Redundancy | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 18 % (w/v) PEG 3350 200 mM lithium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |