4GA0

Structure of the N-terminal domain of Nup358

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-01-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 2
Unit cell lengths	59.219, 82.768, 29.543
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.161 - 1.150
R-factor	0.2157
Rwork	0.214
R-free	0.23930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ga2
RMSD bond length	0.006
RMSD bond angle	1.123
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.200	1.190
High resolution limit [Å]	1.150	1.150
Number of reflections	50246
<I/σ(I)>	25.4	1.7
Completeness [%]	96.7	76.6
Redundancy		7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	18 % (w/v) PEG 3350 200 mM lithium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 277K