4G9O
Crystal Structure of H234A Mutant of Stationary Phase Survival Protein (SurE) from Salmonella typhimurium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-02 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9537 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 81.040, 98.140, 127.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 - 2.120 |
R-factor | 0.22215 |
Rwork | 0.219 |
R-free | 0.27291 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v4n |
RMSD bond length | 0.005 |
RMSD bond angle | 1.003 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.520 | 2.230 |
High resolution limit [Å] | 2.120 | 2.120 |
Rmerge | 0.057 | 0.499 |
Number of reflections | 29206 | |
<I/σ(I)> | 19.1 | 4 |
Redundancy | 6.4 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Under Oil, Microbatch Method | 7.5 | 293 | 0.1M HEPES pH 7.5, 0.02M MgCl2 hexahydrate, 22% (w/v) Polyacrylic acid 5100 sodium salt, Under Oil, Microbatch Method, temperature 293K |