4G97
Crystal structure of the response regulator PhyR from Brucella abortus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.980110 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 53.370, 53.370, 177.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.610 - 2.050 |
| R-factor | 0.2143 |
| Rwork | 0.212 |
| R-free | 0.24690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 3N0R |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.170 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.070 | 1.055 |
| Number of reflections | 16947 | |
| <I/σ(I)> | 19.92 | 1.87 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 8.31 | 8.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.9 | 292 | 22% PEG 4000, 11% ISOPROPANOL, 0.1 M SODIUM ACETATE, pH 8.9, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
