4G96
Crystal structure of calcium2+-free wild-type CD23 lectin domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-14 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.398, 56.728, 62.202 |
| Unit cell angles | 68.49, 88.16, 73.40 |
Refinement procedure
| Resolution | 39.400 - 2.250 |
| R-factor | 0.176 |
| Rwork | 0.173 |
| R-free | 0.23590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h2r |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.944 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.400 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 29893 | |
| Completeness [%] | 94.6 | 86.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 291 | 18.5% PEG 6000, 2% 1,6-hexanediol, 0.05M ammonium sulphate, 0.1M sodium acetate pH 4.7, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
