4G8U

Rat Heme Oxygenase-1 in complex with Heme and O2 with 13 hr illumination: Laser off

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	65.172, 65.172, 120.643
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.670 - 2.100
R-factor	0.15691
Rwork	0.155
R-free	0.18837
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4g7l
RMSD bond length	0.018
RMSD bond angle	2.397
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC (5.6.0117)
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	17780
<I/σ(I)>	10.7
Completeness [%]	99.0	99.1
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	4M sodium formate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K