4G8P
Rat Heme Oxygenase-1 in complex with Heme and CO with 16 hr Illumination: Laser on
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-09 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.033 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 66.021, 66.021, 120.248 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.080 - 1.900 |
R-factor | 0.16833 |
Rwork | 0.166 |
R-free | 0.20935 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4g7u |
RMSD bond length | 0.023 |
RMSD bond angle | 2.462 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC (5.6.0117) |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 24439 | |
<I/σ(I)> | 11.8 | 3 |
Completeness [%] | 99.2 | 98.6 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 4M sodium formate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |