4G8E
Crystal Structure of clone18 TCR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.956 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.420, 84.090, 58.280 |
| Unit cell angles | 90.00, 111.88, 90.00 |
Refinement procedure
| Resolution | 45.480 - 2.200 |
| R-factor | 0.2439 |
| Rwork | 0.241 |
| R-free | 0.29350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nw2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.400 | |
| High resolution limit [Å] | 2.200 | 10.000 | 2.200 |
| Rmerge | 0.048 | 0.022 | 0.235 |
| Number of reflections | 233 | 4208 | |
| <I/σ(I)> | 18.57 | 41.18 | 5.49 |
| Completeness [%] | 95.8 | 95.1 | 86.8 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 25% PEG1500, 10% Proprionate-cacodylate-bis tris propane pH 7, vapor diffusion, hanging drop, temperature 298K |
