4G78
Subatomic Resolution Crystal Structure of Histidine-containing Phosphotransfer Protein MtHPt2 from Medicago truncatula
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-17 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 25.240, 50.150, 50.380 |
Unit cell angles | 90.00, 97.44, 90.00 |
Refinement procedure
Resolution | 50.000 - 0.920 |
R-factor | 0.121 |
Rwork | 0.121 |
R-free | 0.15500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3us6 |
RMSD bond length | 0.023 |
RMSD bond angle | 0.040 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | SHELX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 0.940 |
High resolution limit [Å] | 0.920 | 0.920 |
Rmerge | 0.036 | 0.738 |
Number of reflections | 84634 | |
<I/σ(I)> | 21.78 | 1.89 |
Completeness [%] | 97.8 | 75.1 |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 292 | 0.09M Halogens, 0.1M buffer system 1, 30% PEG 550MME + PEG 20K (Molecular Dimensions Morpheus Screen B1), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |