4G76
Structure of PaeM, a colicin M-like bacteriocin produced by Pseudomonas aeruginosa
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.812, 72.400, 99.693 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.851 - 2.385 |
R-factor | 0.1737 |
Rwork | 0.170 |
R-free | 0.24270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4g75 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.898 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.530 |
High resolution limit [Å] | 2.380 | 2.380 |
Number of reflections | 22837 | |
<I/σ(I)> | 12.7 | 5.2 |
Completeness [%] | 95.7 | 83.5 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100mM Hepes pH7.5; 2M sodium formate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |