4G71
Structure of Recombinant Cytochrome ba3 Oxidase mutant V236N from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 144.440, 98.360, 94.840 |
Unit cell angles | 90.00, 127.81, 90.00 |
Refinement procedure
Resolution | 74.930 - 2.900 |
R-factor | 0.18218 |
Rwork | 0.180 |
R-free | 0.22408 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.666 |
Data reduction software | MOSFLM |
Data scaling software | PROTEUM PLUS (PLUS) |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 74.930 |
High resolution limit [Å] | 2.900 |
Number of reflections | 21974 |
Completeness [%] | 99.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 6.5 | 293 | 50mM Sodium Cacodylate PH 6.5, 1.6M NaCl, 40% PEG400 , Lipidic Cubic Phase, temperature 293K |