4G6V
CdiA-CT/CdiI toxin and immunity complex from Burkholderia pseudomallei
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.92 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 151.963, 173.654, 174.822 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.400 - 2.640 |
R-factor | 0.2065 |
Rwork | 0.204 |
R-free | 0.24520 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.186 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.400 | 2.740 |
High resolution limit [Å] | 2.640 | 2.640 |
Number of reflections | 34071 | |
Completeness [%] | 99.5 | 100 |
Redundancy | 29.6 | 30.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 0.49 M sodium phosphate monobasic, 0.96 M potassium phosphate dibasic, 10 mM triethylene glycol, 504 nM chymotrypsin, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |