4G6D

G1 ORF67 / Staphyloccus aureus sigmaA domain 4 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	70
Detector technology	CCD
Collection date	2009-10-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	38.381, 64.724, 108.721
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.350 - 1.997
R-factor	0.2065
Rwork	0.205
R-free	0.24260
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.431
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.120
High resolution limit [Å]	1.997	1.997
Rmerge		0.879
Number of reflections	18679
<I/σ(I)>		2.75
Completeness [%]	98.3	98.3
Redundancy		5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	0.1M MES pH 6.5, 10% (w/v) PEG5000MME, 12% (v/v) Isopropanol, VAPOR DIFFUSION, SITTING DROP, temperature 295K
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	0.1M MES pH 6.5, 10% (w/v) PEG5000MME, 12% (v/v) Isopropanol, VAPOR DIFFUSION, SITTING DROP, temperature 295K