4G6A
Structure of the Hepatitis C virus envelope glycoprotein E2 antigenic region 412-423 bound to the broadly neutralizing antibody AP33
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-12-02 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.98000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.875, 191.804, 59.374 |
Unit cell angles | 90.00, 111.08, 90.00 |
Refinement procedure
Resolution | 47.970 - 2.501 |
R-factor | 0.1803 |
Rwork | 0.178 |
R-free | 0.21950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dgv |
RMSD bond length | 0.003 |
RMSD bond angle | 0.786 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.970 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 29450 | |
Completeness [%] | 91.3 | 61.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 17% (w/v) PEG 4000, 8.5% (v/v) 2-propanol, 15% (v/v) glycerol and 0.085 M Sodium HEPES, pH 7.5, VAPOR DIFFUSION, temperature 293K |