4G67
Crystal structure of a COG1565 superfamily member and likely methyl transferase from Burkholderia thailandensis bound to S-adenosyl-homocysteine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.977408 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.630, 74.550, 111.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.760 - 1.800 |
R-factor | 0.1605 |
Rwork | 0.159 |
R-free | 0.19820 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.521 |
Data scaling software | XSCALE |
Phasing software | PHASER (2.3.0) |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 1.850 | ||
High resolution limit [Å] | 1.800 | 8.050 | 1.800 |
Rmerge | 0.098 | 0.032 | 0.522 |
Number of reflections | 35828 | 471 | 2397 |
<I/σ(I)> | 13.31 | 33.86 | 2.54 |
Completeness [%] | 99.0 | 99.6 | 91.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 289 | ButhA.17973.a.A1 PS01383 at 20 mg/mL with 2 mM SAH against JCSG+ H7 focus screen, 0.2 M ammonium sulfate, 0.1 M BisTris, 18% PEG 3350 and 15% ethylene glycol as cryo-protectant, crystal tracking ID 234263h3, unique puck ID xgv5-2, pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 289K |