4G5X
Crystal structures of N-acyl homoserine lactonase AidH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 180 |
Detector technology | CCD |
Collection date | 2012-04-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.414, 129.490, 44.759 |
Unit cell angles | 90.00, 111.11, 90.00 |
Refinement procedure
Resolution | 19.870 - 1.290 |
R-factor | 0.1246 |
Rwork | 0.123 |
R-free | 0.15790 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.064 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.290 |
Number of reflections | 124835 |
Completeness [%] | 55.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |