4G5I
Crystal Structure of Porcine pancreatic PlA2 in complex with DBP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-03-25 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 68.860, 68.860, 70.180 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 9.940 - 2.400 |
| R-factor | 0.18511 |
| Rwork | 0.182 |
| R-free | 0.24293 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.984 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.820 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.038 | |
| Number of reflections | 6808 | |
| Completeness [%] | 92.5 | 92.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298.15 | 0.05M Tris maleate buffer, 5mM Calcium chloride, 16% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 290K , pH 7.2, temperature 298.15K |
