4G4R
MutM containing F114A mutation bound to oxoG-containing DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.188, 93.541, 104.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.923 - 1.950 |
R-factor | 0.1738 |
Rwork | 0.172 |
R-free | 0.21420 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.085 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.923 | 2.010 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 31600 | |
Completeness [%] | 90 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 8K, sodium cacodylate, glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |