4G3V
Crystal structure of A. Aeolicus nlh2 gaf domain in an inactive state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.115872 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 65.450, 65.450, 128.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.163 - 1.700 |
| R-factor | 0.165 |
| Rwork | 0.163 |
| R-free | 0.20650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.969 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.042 |
| Number of reflections | 35725 |
| Completeness [%] | 99.6 |
| Redundancy | 10.67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 22% PEG 550 monomethyl ether, 150mM MgCl2, and 100mM HEPES pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
