4G3R
Crystal Structure of Nitrosyl Cytochrome P450cam
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 70 |
Detector technology | CCD |
Collection date | 2012-01-11 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.553, 62.269, 95.490 |
Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
Resolution | 33.100 - 2.200 |
R-factor | 0.193 |
Rwork | 0.190 |
R-free | 0.24990 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.011 |
RMSD bond angle | 1.064 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 33.100 | 33.100 | 2.280 |
High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
Number of reflections | 39581 | ||
Completeness [%] | 97.6 | 99.3 | 92.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 50mM Tris-HCl pH 7.4, 26-32% PEG 4000, 250mM KCl, 1mM D-Camphor, VAPOR DIFFUSION, HANGING DROP, temperature 298K |