4G3Q

Crystal structure of GlmU from Mycobacterium tuberculosis Snapshot 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-02-09
Detector	MAR scanner 345 mm plate
Spacegroup name	H 3
Unit cell lengths	77.070, 77.070, 277.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.810 - 1.900
R-factor	0.172
Rwork	0.171
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.026
RMSD bond angle	2.065
Data scaling software	XSCALE
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.818	1.950
High resolution limit [Å]	1.900	1.900
Rmerge	0.094	0.845
Number of reflections	48337
<I/σ(I)>	17.24	2.84
Completeness [%]	99.7	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	8% PEG 8000, 150MM NACL, 5% GLYCEROL, 1,3-BUTANEDIOL,AMPPNP, MGCL2,0.1M HEPES, COCL2, DTT, VAPOR DIFFUSION, TEMPERATURE 277K, pH 7.5, VAPOR DIFFUSION, SITTING DROP