4G3K
Crystal structure of a. aeolicus nlh1 gaf domain in an inactive state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-21 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 119.350, 119.350, 60.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.910 - 3.050 |
R-factor | 0.212 |
Rwork | 0.208 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.582 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.914 | 3.130 |
High resolution limit [Å] | 3.050 | 3.050 |
Rmerge | 0.101 | 1.392 |
Number of reflections | 8699 | |
<I/σ(I)> | 1.7 | |
Completeness [%] | 99.3 | 99.8 |
Redundancy | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.2 | 293 | 32.5% PEG 3350, 250 mM NaCl, and 50 mM MES pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |