4G2Z
Crystal Structure of C-lobe of Bovine lactoferrin Complexed with Mefenamic acid at 1.90 A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2012-05-26 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.388, 49.830, 65.323 |
Unit cell angles | 90.00, 107.05, 90.00 |
Refinement procedure
Resolution | 19.920 - 1.900 |
R-factor | 0.15007 |
Rwork | 0.148 |
R-free | 0.19124 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ib0 |
RMSD bond length | 0.023 |
RMSD bond angle | 2.096 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.050 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 30529 | |
<I/σ(I)> | 36.5 | 12.5 |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.01M Znso4, 0.1M MES, 25% PEG, Monomethyl Ether 550, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |