4G2A
Crystal structure of a putative nutrient binding protein (lpg2210) from Legionella pneumophila subsp. pneumophila str. Philadelphia 1 at 2.33 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-05-31 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9794,0.9795,0.9184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.953, 87.716, 99.766 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.362 - 2.330 |
R-factor | 0.1702 |
Rwork | 0.168 |
R-free | 0.22170 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.070 |
Data reduction software | XDS |
Data scaling software | XSCALE (March 15, 2012) |
Phasing software | SHELX |
Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.362 | 43.362 | 2.410 |
High resolution limit [Å] | 2.330 | 5.000 | 2.330 |
Rmerge | 0.090 | 0.051 | 0.679 |
Number of reflections | 14552 | 1542 | 1343 |
<I/σ(I)> | 10.09 | 21.7 | 1.85 |
Completeness [%] | 97.5 | 93.2 | 97.5 |
Redundancy | 3.44 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 2.0M ammonium sulfate, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |