4G1V
X-ray structure of yeast flavohemoglobin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-08 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9791 |
Spacegroup name | H 3 2 |
Unit cell lengths | 172.629, 172.629, 82.294 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.100 - 2.098 |
R-factor | 0.1802 |
Rwork | 0.177 |
R-free | 0.24710 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.587 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.6.0095) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.140 |
High resolution limit [Å] | 2.098 | 2.098 |
Rmerge | 0.052 | |
Number of reflections | 27278 | |
Completeness [%] | 99.1 | 99.8 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 2M Ammonium sulphate, 0.1M Tris HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |