4G1I
Structure of the PrgH periplasmic domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2011-05 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97949 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 264.432, 33.380, 48.530 |
Unit cell angles | 90.00, 91.60, 90.00 |
Refinement procedure
Resolution | 45.957 - 1.850 |
R-factor | 0.1758 |
Rwork | 0.174 |
R-free | 0.21390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gr0 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.545 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.048 | 0.495 |
Number of reflections | 43189 | |
<I/σ(I)> | 14.9 | 2.8 |
Completeness [%] | 99.5 | 99.9 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Bicine, pH 8.5, 20% PEG6000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |