4G1F
Crystal Structure of human Dipeptidyl Peptidase IV in complex with a pyridopyrimidinedione analogue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 121.622, 121.362, 143.257 |
| Unit cell angles | 90.00, 114.63, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.900 |
| R-factor | 0.2093 |
| Rwork | 0.207 |
| R-free | 0.25922 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g0g |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.028 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0025) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.890 |
| Number of reflections | 84273 |
| Completeness [%] | 96.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 277 | 22.5% PEG 200mme, 0.1M Bicine pH 7.8 , VAPOR DIFFUSION, SITTING DROP, temperature 277K |
