4G12
Crystal structure of putative TetR family transcriptional regulator, Fad35R, from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-24 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97755 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.836, 83.211, 95.328 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.440 |
R-factor | 0.21301 |
Rwork | 0.208 |
R-free | 0.25596 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.862 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX (Suite (CCP4)) |
Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.500 |
High resolution limit [Å] | 3.440 | 3.440 |
Rmerge | 0.122 | 0.529 |
Number of reflections | 15712 | |
<I/σ(I)> | 12.4 | 2.54 |
Completeness [%] | 99.9 | 98 |
Redundancy | 4.3 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.2M tri-Sodium citrate, 0.1M Tris-HCl pH 8.5, 30%(v/v) PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 298K |