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4G0Z

Crystal structure of Arabidopsis thaliana AGO1 MID domain in complex with GMP

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU MICROMAX-007 HF
Temperature [K]200
Detector technologyCCD
Collection date2010-03-10
DetectorRIGAKU SATURN 944+
Wavelength(s)1.5418
Spacegroup nameP 21 21 21
Unit cell lengths37.291, 60.842, 69.076
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution31.794 - 1.750
R-factor0.1886
Rwork0.187
R-free0.22540
Structure solution methodFOURIER SYNTHESIS
RMSD bond length0.006
RMSD bond angle1.027
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHENIX (1.5_2)
Refinement softwarePHENIX ((phenix.refine: 1.5_2))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]31.7941.810
High resolution limit [Å]1.7501.750
Number of reflections29127
Completeness [%]99.897.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP4.52770.1 M citric acid, 2 M ammonium sulfate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

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