4G0Z
Crystal structure of Arabidopsis thaliana AGO1 MID domain in complex with GMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2010-03-10 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.291, 60.842, 69.076 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.794 - 1.750 |
R-factor | 0.1886 |
Rwork | 0.187 |
R-free | 0.22540 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.027 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.5_2) |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.794 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 29127 | |
Completeness [%] | 99.8 | 97.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 0.1 M citric acid, 2 M ammonium sulfate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |