4G0R
Structural characterization of H-1 Parvovirus: comparison of infectious virions to replication defective particles
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2010-06-04 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9186 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 255.400, 350.400, 271.600 |
| 格子定数 [度] | 90.00, 90.34, 90.00 |
精密化法
| 残基 | 50.000 - 2.700 |
| Rwork | 0.216 |
| R-free | 0.21690 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.365 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.3) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.800 |
| 分解能 [Å] (高) | 2.700 | 2.700 |
| 独立反射数 | 1127217 | |
| <I/σ(I)> | 6.8 | 2.4 |
| 完全性 [%] | 93.1 | 94.7 |
| 冗長性 | 2.6 | 2.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | The reservoir solution contained 1-3% (w/v) polyethylene glycol 8000 (PEG 8000), 150 mM NaCl, and 8mM CaCl2.2H2O as precipitants in 10 mM Tris. The drops were prepared by mixing equivolume of virus solution (10mg/ml) in Tris-HCl Buffer (10 mM Tris-HCl, 150 mM NaCl, 8mM CaCl2.2H2O) with reservoir solution, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
