4G0O

Crystal structure of Arabidopsis thaliana AGO5 MID domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	200
Detector technology	CCD
Collection date	2010-06-08
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	34.823, 37.078, 62.733
Unit cell angles	75.40, 76.06, 90.12

Refinement procedure

Resolution	33.020 - 2.186
R-factor	0.2198
Rwork	0.217
R-free	0.26460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.137
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.7.3_928)
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	16181
Completeness [%]	94.7	88

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	277	0.2M ammonium sulfate, 0.1M sodium citrate, 30% PEG4000, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K